DMEM (High Glucose,L-Glutamine)

Description

Product description

mediumDulbecco’s modified Eagle’s medium (DMEM) One of the most widely used culture media is the modified Eagle medium, which has almost four times the amount of vitamins and amino acids as the base culture medium. In addition, glycine, serine and ferric nitrate have been added. DMEM Medium Suitable for culturing most cell types such as human, hamster, rat, mouse, monkey, chicken and fish cells. Primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, as well as cell lines such as Hela, 293, Cos-7, and PC-12 grow well in this culture medium. Excess glucose has been shown to be suitable for culturing other cell lines, such as primary cultures of mouse and chicken cells, as well as different types of normal cell lines and transformants. Specifically this medium (BI-1001) is a modified form of the basic DMEM medium containing high levels of glucose (4.5 g / L), 15 mM HEPES, L-glutamine, pyruvate, sodium bicarbonate and phenol red. Is. To better maintain the physiological pH at different concentrations of carbon dioxide, HEPES (hydroxyl-piperazine ethane sulfonic acid) solution was added to the medium as a zoetronic chemical buffering agent.

Hints

• Pay attention to product storage conditions
• Do not use the product after the expiration date.
• Store the product in a dark place away from direct light.
• Use the product under sterile conditions (for example under a laminar hood).
• To avoid contamination, use a suitable cover (such as gloves, masks, and sanitary pads) when using the product.
• Supplements such as antibiotics should be added sterilely to the culture medium. Storage conditions and expiration date of the product will change according to the nature of the supplement.
• The culture medium should be clear and free of any suspended particles. Do not use if the environment is cloudy or contains sediment.
• In cases where this culture medium is used several times, note that after opening the crop for the first time, the ratio of air to medium will increase. Therefore, the environment will become alkaline sooner than expected. It is recommended to keep the rest of the environment in sterile 50 ml tubes. In this case, it will be usable until the expiration date on the product.
• To be aware of supplements and specific physiological growth needs for different cell lines, it is recommended to review the articles.
• This product is for research use only.

quality control

• Appearance: red, clear solution
• pH: 4.7 – 6.7
• Sterility: Approved
• Storage conditions: 8-2 ° C, in the dark
• Shelf life: 6 months