HBSS (With HEPES)

Hank’s Balanced Salt Solution (HBSS) was developed by Hanks and Wallace (1949) for use in cells maintained in the less CO2 environment or CO2 free environment. HBSS is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. In summary, the roles of a balanced salt solution are

Maintaining of intra- and extracellular osmotic balanc

• Providing water and inorganic ions that are essential for cells metabolis
• Providing energy for cells metabolis
• Buffering capacity to maintain the physiological pH (6.6 – 6.7) of the environmen

HBSS is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium (BI-1403) are required for rinsing chelators from the culture before cell dissociation. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation

This specification is produced in two different volumes of 100ml (BI-1403-01) and 500ml (BI-1403-05).

Specificatio

The product contains sodium bicarbonate and phenol red.

Without calcium chloride, magnesium sulfate

With HEPES. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), a zwitterionic organic chemical buffering agent is used for better maintaining the physiological pH changes in carbon dioxide concentration

Notes

• Respect storage conditions of the product
• Do not use the product after its expiry dat
• Protect the product from light
• Manipulate the product in aseptic conditions (e.g. under laminar air flow)
• To avoid contamination, wear clothes adapted to the manipulation of the product (e.g. gloves, mask, and hygiene cap
• Supplements, such as antibiotics, should be added as sterile supplements to the medium
• Medium should be clear and free of particulate and flocculent material. Does not use, if the medium is cloudy or contains a precipitat
• In the case of using the medium in several steps, notice that after the first discharge, the air-to-medium ratio will in increase inside. So, the medium will become alkaline earlier than expected. It’s recommended to fill the remaining medium in 50ml sterile tubes and close tightly. Therefore, it can last until the expiry dat
• For research use only.

Quality Control

• Appearance: Red, clear solutio
•  pH= 6.6 – 6.7
• Sterility: tested
• Storage: 2-8° C; Protect from ligh
• Shelf life: 6 months

Inorganic Salts: No Calcium, No Magnesium