DMEM (Low Glucose, L-Glutamine)

Description

Product Descriptio

Dulbecco’s Modified Eagle’s Medium (DMEM) is one of the most widely used modifications of Eagle’s medium that contains approximately four times as much of the vitamins and amino acids present in the original formula. DMEM is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken, and fish cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. This product(BI-1002) is Dulbecco’s Modified Eagle Medium with glucose (low concentration: 1g/L), HEPES (15 mM), L-glutamine, pyruvate, sodium bicarbonate and Phenol Red. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), a zwitterionic organic chemical buffering agent is used for better maintaining the physiological pH changes in carbon dioxide concentrations

Notes

• Respect storage conditions of the product.

• Do not use the product after the expiry date.

• Protect the product from light.

• Manipulate the product in aseptic conditions (e.g. under laminar air flow).

• Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. gloves, mask, and hygiene cap).

• Supplements, such as antibiotics, should be added aseptically to the medium. Storage conditions and shelf-life of the supplemented product would be affected by the nature of the Supplements.

• The medium should be clearand free of particulate and flocculent material. Do not use, if the medium is cloudy or contains a precipitate.

• In the case of using the medium in several steps, notice that after the first discharge, the air-to-medium ratio will in increase inside. So, the medium will become alkaline earlier than expected. It’s recommended to fill the remaining medium in 50ml sterile tubes, close tightly and use until the expiry date.

• Users are advised to review the literature for recommendations regarding medium supplementation and physiologi cal growth requirements specific to different cell lines.

• For research use only.

Quality Contro

• Appearance: Red, clear solution

• pH: 7.40 -7.60

• Sterility: tested

• Storage: 2-8° C;

• Protect from light

•Shelf life: 6 months