L-Glutamine 200mM (100X)

L-glutamine is an essential amino acid required for most cultured cell growth. It is a major source of nitrogen, carbon, and energy. However, misuse of glutamine can be harmful to cells due to ammonia/ammonium ions produced from glutamine. Ammonia has been reported for decades to be toxic and inhibitory to mammalian cell growth and cause reducing cell growth rate, lowering cell densi. y. Ammonia and ammonium ions co-exist in the solution and the relative amount of each species depends on pH of the solution. The main source of ammonia in cell culture is the glutamine through two alternative route: , including metabolic degradation of L-glutamine in the mitochondria, and spontaneous decomposition due to the instability of L-glutamine at physiological pH in liquid media. . The rate of L-glutamine breakdown to the ammonia and pyroglutamate is highly temperature-dependent. The L-glutamine has a range of half-life including 2 months at 4° C and 1 week at 36°. . However, both the powder form and frozen solution are very stable. The recommended concentration of L-Glutamine ranges from 0.5 to 10mM depending on the type of medium.

Specification

• Concentration: 200 mM, X 100
• اProduct Size: 100 mL
• Classification: Animal Origin-Free
• For cell culture applications

quality control

• Appearance: red, clear solution
• Sterility: Approved
• Storag:-5°C to -20°C; Protect from light.
• Shelf life: 12 months
• Shipping condition: : Dry ice

Hints

• Pay attention to product storage conditions.
• Do not use the product after the expiry date.
• L-glutamine may precipitate during the thawing process. The bottle may be placed in a 37ºC water bath and gently swirled during thawing to get L-glutamine back into solution. Do not leave the bottle in the water bath once the prod uct has been thawed. As soon as it melts, remove the solution from the water bath.
• Use the product under sterile conditions (for example under a laminar hood).
• To avoid contamination, use a suitable cover (such as gloves, masks, and sanitary pads) when using the product.
• In order to preserve the quality of the product, it is recommended to thaw out and aliquote the flask in several tubes